Journal: Advanced Science
Article Title: Simultaneous Functional Magnetic Resonance and Optoacoustic Imaging of Brain‐Wide Sensory Responses in Mice
doi: 10.1002/advs.202205191
Figure Lengend Snippet: Hybrid system for concurrent magnetic resonance optoacoustic tomography (MROT) imaging of murine brain activation. a) Illustration showing the imaging performance comparison between fMRI and OAT and the high complementary value of their combination for functional neuroimaging studies. b) Schematics of the hybrid MROT system featuring the 9.4T MRI bore inserted with a customized MRI‐compatible spherical matric array transducer for volumetric data acquisition, a fiber bundle for pulsed light delivery, animal fixation parts, and radiofrequency (RF) coils. c) Electrical stimulation paradigm applied to the left forepaw of a mouse. Stimulation cycle parameters: 0.5 ms pulse duration, 0.5 mA current, 4 Hz pulse repetition frequency, 20 s duration. One stimulation sequence consists of nine stimulation cycles repeated every 100 s. d) Volumetric OAT image of the mouse brain (HbO component is shown). e) The corresponding magnetic resonance angiography (MRA) image acquired with the fast low angle shot (FLASH) sequence. f) Axial and sagittal views of the coregistered OAT and MRA images.
Article Snippet: The hybrid MROT imaging system (Figure ) was based on a customized MRI‐compatible spherical matrix transducer array (Imasonic SAS, Voray, France), MRI‐compatible fiber bundle (CeramOptec GmbH, Bonn, Germany), and a customized RF coil integrated into a 3D‐printed animal holder and inserted into a high‐field MRI scanner (BioSpec 94/20, Bruker BioSpin, Germany).
Techniques: Tomography, Imaging, Activation Assay, Functional Assay, Sequencing